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Dartel, as currently implemented in SPM, will only work using segmented images. If you try with non-segmented scans, the results are unlikely to be good. Best regards, -John On 20 November 2012 09:40, Qasim Bukhari <[log in to unmask]> wrote: > Dear SPM Experts, > I m trying to use DARTEL without segmentation (since I m working with animal > subjects). As far as I have understood is, I did the following steps > 1. Create Template : Here I input the structural images from different > subjects. Should these structural images be coregistered with each other or > not ? > 2. Normalize to MNI : I input the DARTEL template here, Template_6.nii > created from the last step. Though I didnt understand why we have to input > the template_6.nii; what difference would it make if I input template_5.nii > ?? In the subject flow field, I input u_.... created in the last step. In > the Images field, I input the coregistered functional images of the > subjects. I believe I can input functional images of all the subjects at > once; right ??? > So after this it created one mat file, template_6_2mni.mat. I m not sure > what this mat file means. What I was looking for was a number of normalized > images; just like it creates when we do the normal normalization step in > SPM. Can someone please explain this ? > > Thanks a lot > best regards, > Qasim > > Qasim Bukhari > > Research Assistant and Doctoral Candidate > > Institute for Biomedical Engineering > > ETH and University Zurich > > Wolfgang-Pauli-Strasse 27, HIT E22 > > webpage: http://www.micro.biol.ethz.ch/people/sybukhar/index > >