-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1

Dear Jahan,

is your diffraction useless, or do you call 4A resolution useless? 4A is
not too bad and can be a start for structure determination while you are
waiting for better crystals.

You give very little information about what you actually have done, and
if you search the bb-archive for "how to get better crystals" you can
probably make a book from the number of hits.

If you got a large number of crystals while seeding, try harder - the
idea of micro seeding is to adjust the conditions below nucleation
concentration and seed there. Dilute your cat whisker further until you
have only very few seeds left (you may want to have a look at Fig.
11.2.(a) on p. 63 of http://shelx.uni-ac.gwdg.de/~tg/thesis.pdf to get
an idea of the effect.

If all else fails, you can also try restricted proteolysis, although I
would put this in "routine optimization methods" nowadays.

Good luck,
Tim

On 10/16/2012 12:01 AM, Jahan Alikhajeh wrote:

Dear Friends,

I am trying to crystalize a 70 kDa nasty protein but I got plate

shape crystals with high mosaicity and useless diffraction (up to

4A). I tried to improve/optimize crystallization but either I got

the same or nothing. I tried seeding but I had so many crystals

without any improvement. Does anyone have better idea than routine

optimization method in the lab? Thanks in advance.

Jahan

- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen

GPG Key ID = A46BEE1A

-----BEGIN PGP SIGNATURE-----
Version: GnuPG v1.4.12 (GNU/Linux)
Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/

iD8DBQFQfQ68UxlJ7aRr7hoRAqsOAKCI5hIkO2VkI4EGEuQQfggWQASFqQCg2BHo
6QaulOnNvPRocZ9r4BlQTVg=
=FM/p
-----END PGP SIGNATURE-----