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Hi, Thanks, i was thinking of a situation where one can model the sequence to a structure based on homologous structures, ie assuming i know the fold - i guess PISA / ASA based estimates are the first thing. (mainly indeed the likelyhood to aggregate ie the surface property estimates are what i am looking for.) Tommi On Oct 12, 2012, at 8:38 PM, Das, Debanu wrote: > Hi, > > Using a surface property analysis (3D structure is available), I think you can get a quantitative estimate by using PISA to find the solvent-accessible area and salvation energy and then comparing that to corresponding values obtained by using in your lab another protein for calibration (maybe ones that you label as highly, medium or minimal soluble with known mg/ml). > > If you just want an estimate from sequence analysis for solubility on heterologous overexpression, you can try: > SOLpro in http://scratch.proteomics.ics.uci.edu/ > Or > http://mips.helmholtz-muenchen.de/proso/proso.seam > http://www.biotech.ou.edu/ > > Thanks, > Debanu > > > -----Original Message----- > From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Tommi Kajander > Sent: Friday, October 12, 2012 10:23 AM > To: [log in to unmask] > Subject: [ccp4bb] solubility estimates for domains/structures? > > Hi all, > Does anyone a program/paper that would give some quantitative estimate for protein solubility based on surface property analysis? > (excluding obvious things such as integral membrane / TM regions) > > Best > Tommi > > > > > Tommi Kajander, Ph.D., Docent Structural Biology and Biophysics Institute of Biotechnology University of Helsinki Viikinkaari 1 (P.O. Box 65) 00014 Helsinki Finland p. +358-9-19158903 [log in to unmask] http://www.biocenter.helsinki.fi/bi/kajander/Kajander_Lab/Home.html