 |
 |
Hi, Yes, it is worth trying. Nonionic detergents can be good for this. One example I know of and readily comes to mind is the use of 0.1% NP40 (Noniondet P40) in the stabilization of murine reverse transcriptase during purification (also helps toprevent precipitation), first described in: "Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli.", Roth MJ, Tanese N, Goff SP., J Biol Chem. 1985 Aug 5;260(16):9326-35. http://www.ncbi.nlm.nih.gov/pubmed/2410413 DDM may also be good, use at concentration much below CMC. Might be best to check out and screen several detergents, either by mini column purification or by DLS. Thanks, Debanu. -----Original Message----- From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Vitali Stanevich Sent: Friday, October 12, 2012 9:42 AM To: [log in to unmask] Subject: [ccp4bb] off-topic: detergents for the stabilisation of water-soluble proteins Hi, Sorry for off-topic question. Does anyone have experience of the stabilisation of water-soluble proteins by detergents? Protein I'm working with is definitely water-soluble and has high yield, but, unfortunately, not very stable. Especially during concentration. So, we thought that adding some detergents may one of the ways to stabilise protein. So, did anyone do it before or may be know published examples? Any suggestions on the detergent type/concentration would be welcome. Thanks, Vitali