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I saw also a great domain (around 150aa) movement on one of the subunits when crystals of the protein (yeast acetylglutamate kinase) were soaked with its inhibitor (arginine): http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0034734 2012/10/9 Alan Cheung <[log in to unmask]> > Depends on what you mean by large, but we saw very obvious domain movement > when we soaked a protein (transcription factor TFIIS) into crystals of RNA > Polymerase II (PMIDs 12914699/21346759/22726432). We recently animated > this conformational change, and you can watch it at: > > http://www.youtube.com/watch?**v=WlMV_l88Lus<http://www.youtube.com/watch?v=WlMV_l88Lus> > > ...fast forward to about 5 mins 20 second to watch TFIIS binding. And, > er, sorry for the shameless plug ;) > > Alan > > > > > > On 09/10/2012 15:33, WENHE ZHONG wrote: > >> Dear CCP4 members, >> >> Recently, I got a ligand soaking structure to clearly show a large >> domain (~100 amino acids) movement compared to the no soaking structure. >> Although there are some reported examples of this enzyme to suggest the >> flexibility of this large domain which is relevant to substrate binding. >> _But it is the first time I can see it happen in crystal soaking >> procedure._ In this case, I am pleased by this result. >> >> >> My question is, do you have any other example like mine, where domain >> (or loop) movement is observed _*in crystal*_ during ligand _*soaking*_ >> >> procedure? It would be very helpful for me to relate my result to other >> similar examples. Thank you very much. >> >> King regards, >> Wenhe >> > > -- > Alan Cheung > Gene Center > Ludwig-Maximilians-University > Feodor-Lynen-Str. 25 > 81377 Munich > Germany > Phone: +49-89-2180-76845 > Fax: +49-89-2180-76999 > E-mail: [log in to unmask] >