I used the Luminex multiplexing IA platform a few years ago in a previous life for gut hormone/diabetes markers.  I'm sure a single assay could cover the vast majority if not all the markers you list below, I was using rat/mouse plasma at the time and got down to  10 or 20 ul sample volume as far as I can recall for assay of up to 8 different markers.

 

The main problem with the platform, unsurprisingly not noted in the technical details, is that the different beads used for each different marker can cross interfere between assays.  Hence I had to validate eah single bead assay and then make sure that adding all the different beads together didn't then have any effect on one another.

 

It is quite technically challenging, additionally a single 96 well plate assay using multiple beads is expensive (although this wasn't really a problem back in the pharmaceutical industry).

 

Regards

ed

 

From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Mohmed Ashmaig
Sent: 22 October 2012 14:05
To: [log in to unmask]
Subject: Cytokines

 

Dear all,

Is anyone aware of an instrument for measuring cytokines, some people using Luminex and other use ELISA,

Please share your experience with me,

Also we would like to know if any lab in USA can measure cytokines such as;

ICAM-1

IL-1α

IL-6

MCP-2

TNFα

TNF-β

Best regards

Mohmed

 

------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/


This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system.
 ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/