Thank you Mark. That worked. My other scripts on separate dataset were working fine, only this particular script had a glitch. Thanks for your help. Suril On Fri, Sep 7, 2012 at 1:23 AM, Mark Jenkinson <[log in to unmask]> wrote: > Dear Suril, > > I think you have a problem in that you creat func2struct_RESTEC1.mat twice > (both of the two flirt calls at the start use the same output name) so this > file is not the func2struct registration, but actually the struct2standard. > In the fnirt call this is fine (as you should be using the struct2standard > affine here). But in the final applywarp call the --premat really does > need to be the func2struct, which you don't have at that point. > > If you change the names for the affine matrices in steps 1 and 5 (first > flirt call and second applywarp call) then I think this should work. > > All the best, > Mark > > > On 6 Sep 2012, at 19:42, SURIL GOHEL <[log in to unmask]> wrote: > > > Hi FSL experts, > > I am facing troubles with FNIRT registration of my functional data using > anatomical. > > I am using following command lines. > > > `flirt -ref mprage_betted.nii.gz -in fin_RESTEC1_1tp.nii.gz -dof 7 -omat > func2struct_RESTEC1.mat`; > > `flirt -ref /usr/local/fsl/data/standard/MNI152_T1_2mm_brain.nii.gz -in > mprage_betted.nii.gz -omat func2struct_RESTEC1.mat`; > > `fnirt --in=MPRAGE.nii.gz --aff=func2struct_RESTEC1.mat > --cout=struct2standard_fnirt_RESTEC1 --config=T1_2_MNI152_2mm`; > > `applywarp --ref=/usr/local/fsl/data/standard/MNI152_T1_2mm > --in=MPRAGE.nii.gz --warp=struct2standard_fnirt_RESTEC1 > --out=reor_MPRAGE_fnirt`; > > > `applywarp --ref=MNI152_T1_2mm.nii.gz --in=fin_RESTEC1_1tp.nii.gz > --warp=struct2standard_fnirt_RESTEC1 --premat=func2struct_RESTEC1.mat > --out=fin_RESTEC1_1tp_mni3_fnirt`; > > > The problem is anatomical file is fine after registration and matches well > with 2mm MNI brain but my functional scan is not registered. > The functional brain is shifted to the right and only half of the scan is > registered. > > All my data is in RPI orientation. > > The only problem i can think of is that my functional scans are sagittal > scans while my anatomical scans are axial scans. > > Attached are the AFNI screen shots of registered images. > Any help would be greatly appreciated. > > -- > -Suril > 973-972-7076 > > > > <anatomy.png><functional.png> > > >