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I played around and so I found this. In the beginning it was set to both, like it is my default. Anyway, all peak are picked already so this option shouldn't influence the atom type typing. justin On 11/09/12 19:42, Markus Heller wrote: > Err, so the HNCACB has positive and negative peaks ... So why don't you tell Analysis to pick positive *and* negative peaks? > > Markus > > -----Original Message----- > From: CcpNmr software mailing list [mailto:[log in to unmask]] On Behalf Of Justin Lecher > Sent: Tuesday, September 11, 2012 10:26 AM > To: [log in to unmask] > Subject: Re: Add CO/HA/CA/CB only work for CB?? > > On 11/09/12 18:58, Justin Lecher wrote: >> Hello, >> >> doing a standard seq. assignment with an HNcoCACB as query and an HNCACB >> as match. Both spectra have root dims assigned. If I now do a sequential >> link, the non root resonance gets created as expected. But when I click >> "Add...." only the CB is set, but the resonance which should be CA is >> removed again. Was this observed before? >> >> Thanks, >> Justin >> > > Okay, > > I found something "bad". > If I set "pick only positive peaks", the CB are marked as CB and the CA > are omited. In contrast, setting "pick negative only" brings the CB > assignement to the CA and the CB are omited this time. This is wrong. > Picking both, lets all peaks be unassigned. > > If I compare my spectra with the tutorial ones, the difference in the > signs of the peaks e.g. CB(tutorial) = -1 * CB(justin). > Is this fixable? > > Justin > > > -- Justin Lecher Institute of Complex Systems ICS-6 Structural Biochemistry Research Centre Juelich 52425 Juelich, Germany phone: +49 2461 61 2117