Thanks Zhijie,<br><br>So do you mean to say that your lab regularly uses it in Lysis and refolding buffers, and the protein/s purified are structurally and functionally fine and that there is no aggregation caused due to sarkosyl??<br>
<br>kind regards<br>Rashmi<br><div class="gmail_quote">On Thu, Sep 20, 2012 at 6:23 PM, Zhijie Li <span dir="ltr">&lt;<a href="mailto:[log in to unmask]" target="_blank"><a href="/cgi-bin/wa-jisc.exe?LOGON=A3%3Dind1209%26L%3DCCP4BB%26E%3Dquoted-printable%26P%3D1553797%26B%3D--f46d042ef475986b1f04ca2146df%26T%3Dtext%252Fhtml%3B%2520charset%3DISO-8859-1%26pending%3D" target="_parent" >[log in to unmask]</a></a>&gt;</span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
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<div><font face="Arial">Hi,</font></div>
<div><font face="Arial"></font> </div>
<div><font face="Arial">We used it in some lysis/refolding 
buffers.</font></div>
<div><font face="Arial"></font><font face="Arial"></font> </div>
<div><font face="Arial">Sigma marked this biodegradable detergent 
as &quot;Highly toxic by inhalation&quot; - when it&#39;s used in toothpastes and 
shampoos if I am not mistaken.  when I called, Sigma said that since 
someone published a paper saying that when they sprayed it into a rat&#39;s nose the 
rat died, they had no choice but to put a skull sign on the MSDS.</font></div>
<div><font face="Arial"></font> </div>
<div><font face="Arial">Zhijie</font></div>
<div style="FONT:10pt Tahoma"><font face="Arial"></font><font face="Arial"></font><font face="Arial"></font><font face="Arial"></font><font face="Arial"></font> </div>
<div style="FONT:10pt Tahoma"><font face="Arial"></font> </div>
<div style="FONT:10pt Tahoma">
<div><br></div>
<div style="BACKGROUND:#f5f5f5">
<div><b>From:</b> <a title="mailto:[log in to unmask]
CTRL + Click to follow link" href="mailto:[log in to unmask]" target="_blank">Rashmi Panigrahi</a> </div>
<div><b>Sent:</b> Thursday, September 20, 2012 5:47 AM</div>
<div><b>To:</b> <a title="mailto:[log in to unmask]
CTRL + Click to follow link" href="mailto:[log in to unmask]" target="_blank"><a href="/cgi-bin/wa-jisc.exe?LOGON=A3%3Dind1209%26L%3DCCP4BB%26E%3Dquoted-printable%26P%3D1553797%26B%3D--f46d042ef475986b1f04ca2146df%26T%3Dtext%252Fhtml%3B%2520charset%3DISO-8859-1%26pending%3D" target="_parent" >[log in to unmask]</a></a> </div>
<div><b>Subject:</b> [ccp4bb] sarkosyl</div></div></div><div><div class="h5">
<div><br></div>Hi All,<br>I was wondering if anyone has used N-lauryl sarcosine 
in the lysis buffer for purifying protein and checked if the protein was 
functional or structurally okay<br>Please let me know <br clear="all">kind 
regards<br>-- <br>rashmi<br></div></div></div>
</blockquote></div><br><br clear="all"><br>-- <br>rashmi<br>