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Hi Theresa, You can use our free Discovery Studio Visualizer: http://accelrys.com/products/discovery-studio/visualization-download.php You can select the region of choice (in your case the transmembrane domain) and create a surface around it (Structure -> Surface -> Add). Then in the Molecular Data Table's Surface Tab (View -> Data Table), you can find values for the Surface Area as well as Volume. Let me know if you have any trouble with this. Disclaimer: I work for Accelrys. Cheers, Francisco Sr. Product Manager Accelrys.com -----Original Message----- From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Theresa Hsu Sent: Thursday, September 06, 2012 12:59 AM To: [log in to unmask] Subject: [ccp4bb] Protein volume Dear all I have two membrane protein structures. Is there any tool to calculte the volume of transmembrane domain and solublle domain separately for comparison? Thank you.