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Thanks Zhijie, So do you mean to say that your lab regularly uses it in Lysis and refolding buffers, and the protein/s purified are structurally and functionally fine and that there is no aggregation caused due to sarkosyl?? kind regards Rashmi On Thu, Sep 20, 2012 at 6:23 PM, Zhijie Li <[log in to unmask]> wrote: > ** > Hi, > > We used it in some lysis/refolding buffers. > > Sigma marked this biodegradable detergent as "Highly toxic by inhalation" > - when it's used in toothpastes and shampoos if I am not mistaken. when I > called, Sigma said that since someone published a paper saying that when > they sprayed it into a rat's nose the rat died, they had no choice but to > put a skull sign on the MSDS. > > Zhijie > > > > *From:* Rashmi Panigrahi <[log in to unmask]> > *Sent:* Thursday, September 20, 2012 5:47 AM > *To:* [log in to unmask] > *Subject:* [ccp4bb] sarkosyl > > Hi All, > I was wondering if anyone has used N-lauryl sarcosine in the lysis buffer > for purifying protein and checked if the protein was functional or > structurally okay > Please let me know > kind regards > -- > rashmi > -- rashmi