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Dear SPM Experts, I am wondering what the consequences are of running realignment on already realigned data in my particular case. The data set I am using has been preprocessed before using SPM, but I am redoing it by starting with the same original .img/.hdr files used before (deleting the smoothed and warped files prepended with 's' and 'sw' and using the files that are ostensibly unaltered by preprocessing). The option to create a mean image only was selected during realignment and no other new files (such as those appended with the 'r' prefix or any .mat files) were created in the input folder during either preprocessing attempt. I am wondering if this realignment process in SPM overwrites the original .img/.hdr files in any way and what the consequence of *re-*realigning images (accidental or otherwise) would be downstream in the analysis in this case (e.g. added step of interpolation, reduced spatial resolution, or perhaps something even less desirable of which I am unaware). Thank you for your help, Jared _______________________________ Jared Torre Doctoral Student UCLA Department of Psychology 1285 Franz Hall [log in to unmask] | 818.642.2907