Hi all, I have been trying to reduce the spm affine registration procedures for a 2D application and I have the following doubt: If Mg and Mf are the matrices defining the geometry of the reference (g) and floating (f) image, and if M is the matrix obtained by the registration procedure, what matrix combination should I provide to the reslice routine to transform f in the space of g with M: would it be inv(Mg) * M * Mf ? Also, if the dimensions of the image are in microns (and mm, as they are in neuroimaging) should I be creating Mg and Mf in any special way? Thanks a lot, Sid.