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Hi - yes in general that all should be fine - but of course subject to the normal caveats regarding checking the quality of your registrations and the study-appropriateness of a priori template/atlas. Cheers. On 3 Aug 2012, at 18:17, Hidayath Ansari wrote: > Hi all > > I've just recently started using FSL and there are a few things I'm not quite clear on. I'm processing a DTI dataset (FA maps for now) for machine learning and I'd like to make sure I have things in the right space before going further. I ran TBSS using FMRIB58_FA_1mm as the target for registration. Their website says: > > The FMRIB58_FA_1mm image is in the same space as the MNI152 standard space image > > The JHU atlas website says: > > In the ICBM-DTI-81 white-matter labels atlas, 50 white matter tract labels were created by hand segmentation of a standard-space average of diffusion MRI tensor maps from 81 subjects. > > Given that these two are both in "standard space" aka MNI space - am I correct in assuming that the coordinates I derive from the atlases will map correctly onto my TBSS-processed FA images? In the sense that if the atlas says such and such coordinate belongs to the Cingulum area of the brain, that same coordinate will also correspond to the Cingulum area in my registered FA maps? > > I think it might but now I'm asking myself whether I need an additional warping or registration step. > > I will appreciate any help. > > Thank you! > > Hidayath > University of Wisconsin-Madison --------------------------------------------------------------------------- Stephen M. Smith, Professor of Biomedical Engineering Associate Director, Oxford University FMRIB Centre FMRIB, JR Hospital, Headington, Oxford OX3 9DU, UK +44 (0) 1865 222726 (fax 222717) [log in to unmask] http://www.fmrib.ox.ac.uk/~steve ---------------------------------------------------------------------------