Not much info to go by...

Anyway, if the program 'goes crazy' you either have very different exposure levels, radiation damage leading to non-isomorphism, or you have a trigonal space group (P3xxx or P6xxx) and forgot to make sure all batches are indexed the same way.

If you have translated your crystal between batches, HKL2000 won't of course be able to process all batches in one go. If you haven't touched the crystals at all, and alln-in-one processing doesn't work, the parameters at the end of one batch may not be accurate to start off a new batch, which is mostly due to inaccurate goniostats. In that case, you will need to process the batches individually and them combine them during scaling.

Hope that helps.

MM

On Aug 1, 2012, at 8:50 AM, Uma Ratu wrote:

> Dear All:
>
> I collected 5 data sets from one crystal and would like to process them together.
>
> Here is how I did:
>
> In HKL2000, load the all data sets. "Index" each set. When I try "Intergrate", the program automatically go through the whole data sets there, and do not go through.
>
> I then process data sets by loading one at each time. Index, intergrate and scale all go through very smoothly. But when I put them together, the program just goes crazy.
>
> Thank you for advice
>
> Uma