How about just co-transfecting with two different plasmids which have different selection markers? I&#39;ve done it a lot, and it seems to work fine...<div><br></div><div>JPK<br><br><div class="gmail_quote">On Fri, Aug 24, 2012 at 10:39 PM, Lye, Ming <span dir="ltr">&lt;<a href="mailto:[log in to unmask]" target="_blank"><a href="/cgi-bin/wa-jisc.exe?LOGON=A3%3Dind1208%26L%3DCCP4BB%26E%3Dquoted-printable%26P%3D5910375%26B%3D--047d7b66f839b527f104c83f577b%26T%3Dtext%252Fhtml%3B%2520charset%3DISO-8859-1%26pending%3D" target="_parent" >[log in to unmask]</a></a>&gt;</span> wrote:<br>
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Dear CCP4bb,<br>
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We would like to co-express proteins under Se-Met conditions for de-novo phasing of a complex. One of the proteins expresses much better with a GST-tag compared with a his-tag.<br>
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As its binding site is at its N-terminus, we are hoping to co-express it as a C-terminal GST tag recombinant. So far however, we haven&#39;t found any commercially available duet vectors with such a tag. We would truly appreciate if anyone knows of the availability of such vectors, or has any suggestions on similar vectors.<br>

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Thanks so much!<br>
<span class="HOEnZb"><font color="#888888">Ming<br>
</font></span></blockquote></div><br><br clear="all"><div><br></div>-- <br>*******************************************<br>Jacob Pearson Keller<br>Northwestern University<br>Medical Scientist Training Program<br>email: <a href="mailto:[log in to unmask]" target="_blank"><a href="/cgi-bin/wa-jisc.exe?LOGON=A3%3Dind1208%26L%3DCCP4BB%26E%3Dquoted-printable%26P%3D5910375%26B%3D--047d7b66f839b527f104c83f577b%26T%3Dtext%252Fhtml%3B%2520charset%3DISO-8859-1%26pending%3D" target="_parent" >[log in to unmask]</a></a><br>
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