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Quite common. And it may be a good sign for crystallizability in those conditions. As the concentration gradients dissipate from the initially mixed drop, your protein re-solubilizes. Roger Rowlett On Aug 17, 2012 5:31 PM, "Yuri Pompeu" <[log in to unmask]> wrote: > Hello everyone, > I have just made a curious observation. > I purified an enzyme domain and concentrated it to around 16 mg/mL without > much mischief. > I then proceeded to set-up some drops. After 5 min most of the drops had a > muddy appearance to them which led me to think I was probably too > concentrated in either protein and/or precipitant. > Curiously I went back to look at the drops after an additional 30 min and > they all look pretty clear with no appreciable precipitation. > Has anyone encountered this situation or a similar one before? > Any input/shared experience is welcome. > Best, > Yuri >