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Dear SPMers: I have a very beginner question. My fMRI data is acquired using the bottom-up interleaved slice sequence and the number of slice is 31. The slice sequence is [1:2:31 2:2:31], in the slice timing correction I used slice 31 as my reference slice(which acquired in the middle time of scanning). Now in the specify the 1st level I am confused with the two parameters. They are microtime resolution and microtime onset. Which one of the following is the most suitable one for my experiment, 1). Microtime resolution:31 Microtime onset: 16 2). Microtime resolution:31 Microtime onset: 31 3). Microtime resolution:16 Microtime onset: 1 According to my understanding, I think the first one is the most reasonable parameters. Since I did the slice timing correction, the Microtime resolution should be the number of the slice which is 31. Then because my reference slice is 31 which is the middle one in the acquisition sequence so the microtime onset should be the corresponded time bin of this slice(31) which is 16. Is this right? Hope some experienced SPMers can help me figure this out. Many thanks. Best wishes. Xinyi