Hi Stam, i executed bedpostx_datacheck on the bedpostx directory and the following is the log on the console =================================== /Space/jay/DTI_PWD_baseline_JA_bedpostX/d103/data data_type FLOAT64 dim1 180 dim2 180 dim3 75 dim4 31 datatype 64 pixdim1 1.3329999447 pixdim2 1.3329999447 pixdim3 2.0000000000 pixdim4 1.0000000000 cal_max 0.0000 cal_min 0.0000 file_type NIFTI-1+ /Space/jay/DTI_PWD_baseline_JA_bedpostX/d103/nodif_brain_mask data_type INT16 dim1 180 dim2 180 dim3 75 dim4 1 datatype 4 pixdim1 1.3329999447 pixdim2 1.3329999447 pixdim3 2.0000000000 pixdim4 1.0000000000 cal_max 0.0000 cal_min 0.0000 file_type NIFTI-1+ num lines in /Space/jay/DTI_PWD_baseline_JA_bedpostX/d103/bvals 31 num words in /Space/jay/DTI_PWD_baseline_JA_bedpostX/d103/bvals 31 num lines in /Space/jay/DTI_PWD_baseline_JA_bedpostX/d103/bvecs 31 num words in /Space/jay/DTI_PWD_baseline_JA_bedpostX/d103/bvecs 93 =================================== when i created bedpostx directory, i used whole brain binary mask bedpostx <indir> [-n 2] [-w 1] [-b 1000] but later when i run qboot, i used probability mask(Right_Accumbens) extracted from the standard H-O atlas qboot -k data.nii.gz -m Right_Accumbens.nii.gz -r bvecs -b bvals BW Jay