I'm just about done picking my HNCACB, CBCAcoNH, HNCO, and HNcaCO spectra of a protein with ~ 19 kDa, and ~ 160 AA.  The CBCAcoNH and HNCACB look good, except for three or four regions where three or four signals overlap.

I'm curious to hear what approach people prefer for the backbone assignment and why: the automated bb assignment or the link sequential spin systems?  Or maybe a combination?

I didn't find any comments on the web/in the docu on how to combine the approaches, i.e. use automated assignment for the easy residues and the semi-automated for the trickier ones ...

Thanks for your comments and have a nice weekend!

Markus