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Hi Markus,

Personally, I prefer to do semi-automated assignment and then use automated assignment as a sort of check.  Given the size of your protein, automated assignment could give a reasonable answer, although I would think that you could get 80% of that assigned inside a week using semi-automated assignment and then use automated assignment to see if it can fill in any gaps.

Hope this helps.

Regards

Simon Skinner

On 07/14/2012 08:47 AM, Markus Heller wrote:
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Hello all,

I'm just about done picking my HNCACB, CBCAcoNH, HNCO, and HNcaCO spectra of a protein with ~ 19 kDa, and ~ 160 AA.  The CBCAcoNH and HNCACB look good, except for three or four regions where three or four signals overlap.

I'm curious to hear what approach people prefer for the backbone assignment and why: the automated bb assignment or the link sequential spin systems?  Or maybe a combination?

I didn't find any comments on the web/in the docu on how to combine the approaches, i.e. use automated assignment for the easy residues and the semi-automated for the trickier ones ...

Thanks for your comments and have a nice weekend!
Markus

-- 
Simon P Skinner
Protein Chemistry Group
Leiden Institute of Chemistry, Universiteit Leiden
Phone: +31 71 527 6089 / Fax: +31 71 527 4349
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