As I recall, I used to wear latex gloves, add extra padding to the handle end of the wand (and other tools) with bits of tubing, and take my tools out of the cold room every 20 min or so to de-ice and dry them. I didn’t really have a choice about the cold room because it was the only place my crystals would grow, and my crystallization solution was 20% isopropanol which was too volatile to work with outside the cold room anyway.
Evette S. Radisky, Ph.D.
Assistant Professor
Mayo Clinic Cancer Center
Griffin Cancer Research Building, Rm 310
4500 San Pablo Road
Jacksonville, FL 32224
(904) 953-6372
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Bosch, Juergen
Sent: Friday, July 13, 2012 8:27 PM
To: [log in to unmask]
Subject: Re: [ccp4bb] harvesting in cold room (was: cryo for high salt crystal)
This is a very interesting topic I have to say.
But what I missed in this discussion is the pain you go through when freezing in the cold room. As the name implies it's supposed to be cold (most of the times). But that's not too much of an issue as you can dress up accordingly. The problem I always had was freezing up of the <advertisement> Hampton Magnetic Wand </advertisement> and icing up towards your fingertips after some time when moisture from the cold room condenses and freezes. I hate wearing gloves when handling crystals so there was not much of a skin protection.
How do you guys solve this problem ?
......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-2926
http://lupo.jhsph.edu
