check this article its quite usefulhttp://www.ncbi.nlm.nih.gov/pubmed/12711344--On Wed, Jul 4, 2012 at 4:09 PM, amro selem <[log in to unmask]> wrote:
hallo every body, i face problem with protein when i make gel filtration or concentrate my protein . i notice sever aggregation . i use only glycerol as additives to 50 mM TRIS and 150 mM NaCL ; PH 8 . my IP is 7.8 . any suggestions.best regardsAmr
B. Anuradha
Research scholar,
CAS in crystallography and Biophysics,
University of Madras.