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Hi all- I have a protein that crystallizes in I422, and diffracts well, between 1.3-1.7A. Beautiful density, slightly higher final R-factors than you might expect at this resolution (low to mid 20s). The density is all beautiful, except that I have this one little clash, between a few atoms from a tyrosine and its symmetry mate. In this picture I have it modeled as an Alanine and you can see the two tyrosine rings interlocking; and there is clearly no alternate conformation. [cid:image003.png@01CD5F7B.1C822600] Since it is not near my site of interest, I have been pretty much ignoring it, going through refinement with it as an alanine, then changing it at the very end to a tyrosine and just minimizing B-s, no positional. Now that I plan to publish a bunch of these, I should probably figure out what is really going on. Any insights? Thanks Christine Christine Lukacs Roche This message is intended for the use of the named recipient(s) only and may contain confidential and/or proprietary information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited.