Rex,
\begin{shameless self-advertizing}
Once upon a time we did a systematic study how to optimize crystal size. The study was done with knowledge of the protein solubility (which is not normally available, of course) and we showed that you can still do it when you do not have this information.
Perhaps worth reading. The paper does not end with a shocking conclusion, but more or less with "if you do a systematic study, you can improve crystal volume".
http://www.springerlink.com/content/16133650m43x013g/?MUD=MP
\end{shamelss self-advertizing}
Mark
-----Original Message-----
From: REX PALMER <[log in to unmask]>
To: CCP4BB <[log in to unmask]>
Sent: Wed, Jul 4, 2012 3:55 am
Subject: [ccp4bb] getting larger protein crystals
Does anyone have any new tips/methods for improving crystal size once the initial conditions have been established?
Rex Palmer
http://www.bbk.ac.uk/biology/our-staff/emeritus-staff
http://rexpalmer2010.homestead.com
