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check this article its quite useful http://www.ncbi.nlm.nih.gov/pubmed/12711344 On Wed, Jul 4, 2012 at 4:09 PM, amro selem <[log in to unmask]> wrote: > hallo every body, i face problem with protein when i make gel filtration > or concentrate my protein . i notice sever aggregation . i use only > glycerol as additives to 50 mM TRIS and 150 mM NaCL ; PH 8 . my IP is 7.8 . > any suggestions. > best regards > > Amr > > > > > > > -- B. Anuradha Research scholar, CAS in crystallography and Biophysics, University of Madras.