Hi,

The idea used by the Qiagen pre-screen might be relevant to your case:

http://www.qiagen.com/products/protein/crystallization/screeningproteincrystallizationconditions/prescreenassay.aspx

http://www.qiagen.com/literature/handbooks/literature.aspx?id=2000039

Zhijie

From: [log in to unmask] href="mailto:[log in to unmask]">fengjuanlv12

Sent: Monday, June 11, 2012 3:17 AM

To: [log in to unmask] href="mailto:[log in to unmask]">[log in to unmask]

Subject: [ccp4bb] protein precipitate in all PEG kit

hi all,

I got a protein with good condition without precipitating in the purification process.And the protein is stalbe at 4^oC, but when i try crystallization screens, it precipating in all the kit(with PEG), in the kit with high concentration of salt ,the situation was better(but still no crystal).

any suggestion?

thanks!

fjlv

2012-06-11

fengjuanlv12