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Dear All, I am looking for a bit of advice, and am interested to hear about experiences people have had with various commercially-available 96- well plate-based PCR, plasmid, and protein purification appliances and plates. Forgive me for comparing commercial vendors. We have a 96-well plate-compatible vacuum manifold (Qiavac). However, the plasmid-purification kits from this vendor do seem comparatively pricey to me. So, I am looking for a more cost-efficient system. How compatible are 96-well PCR purification plates/plasmid-binding and filter plates from different vendors with this type of vacuum manifold? From what I can see, it becomes difficult to avoid cross- contamination between wells, when using e.g. Whatman-type 96-well microplates with Qiavac, because the distance between the drip director of the DNA-binding/filter plate and a receiver plate (e.g. PCR plate) in the manifold is relatively long, and the alignment isn't great (drops end up on the rim). The alternative would be centrifugation directly on top of a receiver plate, but I am concerned about cross-contamination. Which type of membranes/filter plates were people most pleased with for (bacterial) lysate-clearing, avoiding clogging with lysates from ~ 5 ml bacterial cultures; and which DNA/plasmid-binding plates have most binding capacity and result in highest purity? Again cost- efficient vendors would be preferred. Regards, Florian