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Hi Sonali, You could try wide-search MR: https://portal.sbgrid.org/d/apps/wsmr/ Best of luck, val On 20 June 2012 19:13, sonali dhindwal <[log in to unmask]> wrote: > Dear All, > > I am working on a protein for last so many years and for which i have got > crystal now in a tray which i kept 1 years ago. It diffracts well and > resolution is 2.2A, which is good. > > I indexed in HKL2000, mosflm and automar and it shows P21 space group in > all data reduction packages. But when I tried using molrep or phaser then I > do not get any solution. The sequence of my protein is having 46% identity > with other available crystal structure. > Also when I tried to get matthews coffecient, it calculates its molecular > mass less ( about 35 kDa) than which should be (original 54kDa) with > solvent content 47%. > > I have also run the silver staining gel of the protein which contained > crystal that shows about 45 kD protein band which is 10 less than the > original. Also I tried to run gel on crystal but it did not give anything > as it was a small crystal. > > I have tried all combinations of the search model and tried to break > available pdb many ways to make different search models but have not got > any good solution. Molrep gives contrast even 10 or more but no good > electron density map yet. Free R and figure of merit becomes 52% and 42% > respectively in Refmac with all the solutions. > > I will highly appreciate all the suggestions for this kind of problem. > > Thanks and regards > > -- > Sonali >