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Hi, Try modeling a zinc close to the histidine and then do one round of refinement the density will improve close to that area, generally a zinc ion coordinated to histidine also has a water molecule close to the zinc. HTH, Shya On Tue, Jun 19, 2012 at 5:47 AM, Lianying Jiao <[log in to unmask]> wrote: > Dear all, > > As in *Screenshot2.png*, some unknown density exists around a histidine. > The crystallization condition contains Zn(OAc)2, PEG3350, pH4.5. Do anyone > tell me what is it? Thanks in advance. > > Sincerely, > Lianying Jiao >