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Hi, Weina, So far as I know, the segmentation is usually automatically done by SPM, no need to set threshold for gray or white matter, or you simply follow the default method. You click the 'Segmentation' in SPM, while select files of T1 images in the data selection. You may also like to choose to keep native-space or normalized grey matter. Good luck! Gao On 21 May 2012 15:26, ÀîÎÀÄÈ <[log in to unmask]> wrote: > Hi every one, > > How much should I set about the threshold for gray matter when doing > segmentation? > > Weina > Hi every one, > > How much should I set about the threshold for gray matter when doing > segmentation? > > Weina > > Hi every one,****** > > **** > > How much should I set about the threshold for gray matter when doing > segmentation in spm?**** > > **** > > Weina > >