Hi All,
Sorry for this off topic.
I am stuck with a plant protein which is not crystallizing. I am expressing this in E coli.
I have done the CD and thermal melt of the deletion constructs of this protein @ 0.2 mg/ml.
Please refer to the doc file attached to this email.
The CD looks okay but the thermal melt looks kind of wierd. I would expect a sigmoid curve for the thermal melts and then when we do a reverse temperature scan, the protein should denature and may not fold back (with some exceptions)
Please let me know if any one had the same experience before and could explain me what this meant.
Kind regards
Anita
