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Gene synthesis and make your own! Tony. Sent from my iPhone On 24 May 2012, at 20:42, "Gloria Borgstahl" <[log in to unmask]> wrote: > My fellow crystallographers, > We are thinking the SUMO/His vectors would be nice to have in the lab > aresenal... but. The stumbling block is that the protease needed for > cleavage is very expensive at crystallography scale. SUMO(ULP-1) > protease costs ~$700/mg fusion protein. It would not be a problem > for labs using micrograms of protein, but is prohibitive at our level > of protein purification. > Has anyone found a way around this? Your pal, Gloria