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Did you locate the Iodine(s)? Did you have iodotyrosine, or I^- bound at anion binding sites? There are two distinct methods based on I2/I-. Florian Sauer wrote: > Dear Rajesh, > > another method to incorporate Iodine into your crystal is by simply > placing a drop of KI/I2 solution next to the crystallization drop. > > Have a look here: > > Acta Cryst. (2006). D62, 280-289 > New methods to prepare iodinated derivatives by vaporizing iodine > labelling (VIL) and hydrogen peroxide VIL (HYPER-VIL) > > Worked for me with salt concentration sensitive crystal and a > derivatization time <10 min. > > Good luck, > > Florian > > > > > Am 03.05.12 15:51, schrieb Rajesh Kumar: >> Dear All, >> >> I have very thin crystals but diffracting. I was not able to handle >> them easily for iodide soak. I always lost the crystals during >> manipulation and other big crystals obtained after seeding doesn't >> even give any diffraction. I tried for co-crystallizing with NaI. The >> crystals appear only up to 20 mM in 1:2 (3ul drop of 1 ul protein and >> 2ul reservoir). >> Is this concentration of iodide is enough for SAD data ( if it had >> good incorporation) ? >> I appreciate your help. >> >> Thanks >> Rajesh >