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Dear Rajesh,

another method to incorporate Iodine into your crystal is by simply placing a drop of KI/I2 solution next to the crystallization drop.

Have a look here:

Acta Cryst. (2006). D62, 280-289
New methods to prepare iodinated derivatives by vaporizing iodine labelling (VIL) and hydrogen peroxide VIL (HYPER-VIL)

Worked for me with salt concentration sensitive crystal and a derivatization time <10 min.

Good luck,

Florian




Am 03.05.12 15:51, schrieb Rajesh Kumar:
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Dear All,

I have very thin crystals but diffracting. I was not able to handle them easily for iodide soak. I always lost the crystals during manipulation and other big crystals obtained after seeding doesn't even give any diffraction. I tried for co-crystallizing with NaI. The crystals appear only up to 20 mM in 1:2 (3ul drop of 1 ul protein and 2ul reservoir).
Is this concentration of iodide is enough for SAD data  ( if it had good incorporation) ?
I appreciate your help.

Thanks
Rajesh