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Gloria, Why don't you introduce TEV-cleavage site? You can clone it between His- and sumo-protein_of_interest (if you want co-crystallise sumo with your protein) or between His-sumo and protein_of_interest (if you want to crystallise your protein only). Vitali On Thu, May 24, 2012 at 2:41 PM, Gloria Borgstahl <[log in to unmask]>wrote: > My fellow crystallographers, > We are thinking the SUMO/His vectors would be nice to have in the lab > aresenal... but. The stumbling block is that the protease needed for > cleavage is very expensive at crystallography scale. SUMO(ULP-1) > protease costs ~$700/mg fusion protein. It would not be a problem > for labs using micrograms of protein, but is prohibitive at our level > of protein purification. > Has anyone found a way around this? Your pal, Gloria >