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Works well on Abbott Architect as well. We switched to Ark assay as wel. Bart Ballieux Leiden University Medical Centre Netherlands -----Original Message----- From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Iqbal, Jhangir Sent: dinsdag 22 mei 2012 13:24 To: [log in to unmask] Subject: Re: Methotrexate assay Works well on Beckman Coulter DXC800 analyser LoQ 0.04 umol/l reproducible Very good comparison between Abbott TDx methotrexate and Ark methotrexate, has been in routine use for 8 months. Best wishes Jhangir Mr Jhangir Iqbal Principal Clinical Biochemist The Royal Marsden NHS Foundation Trust Biochemistry Department Sutton, Surrey Tel: 020 8661 3207 -----Original Message----- From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Ivison Fiona (RW3) CMFT Manchester Sent: 22 May 2012 12:13 To: [log in to unmask] Subject: Re: Methotrexate assay I can share some data/information on this assay which we have been running routinely for at least 6 months on Cobas 6000 instruments. Fiona Dr Fiona Ivison Principal Biochemist Central Manchester Foundation Trust Oxford Rd manchester M13 9WL 0161 701 1203 07806 883960 ________________________________ From: Clinical biochemistry discussion list [[log in to unmask]] on behalf of [log in to unmask] [[log in to unmask]] Sent: 22 May 2012 11:43 To: [log in to unmask] Subject: Vb: Re: Methotrexate assay Has anyone evaluated the Ark-Methotrexate with claimed LoQ 0,04 umol/L? On what platform? Experiences? http://www.ark-tdm.com/DB_methotrexate.html Regards Goran Brattsand, MD, Ph D Clinical chemistry NUS S-901 85 Umea Sweden ----- Vidarebefordrat av Göran Brattsand/US/VLL/SE 2012-05-22 12:30 ----- Från: "Miller,James J." <[log in to unmask]> Till: [log in to unmask] Datum: 2012-05-21 17:15 Ärende: Re: Methotrexate assay ________________________________ Mohammed El Sammak asked about more sensitive methotrexate assays since the discontinuation of the TDx. Saeed Jortani and colleagues just published a modification of the Siemens Viva E methotrexate assay to improve the low end sensitivity. The citation and abstract are below. -Jim Therapeutic Drug Monitoring Issue: Volume 34(2), April 2012, p 193197 Copyright: © 2012 Lippincott Williams & Wilkins, Inc. Publication Type: [Original Article] DOI: 10.1097/FTD.0b013e31824b93a5 ISSN: 0163-4356 Accession: 00007691-201204000-00014 Keywords: methotrexate, EMIT, sensitivity Improved Sensitivity for Methotrexate Analysis Using Enzyme Multiplied Immunoassay Technique on the Siemens Viva-E Instrument Borgman, Mark P. PhD*; Hiemer, Mary F. MT(ASCP)*; Molinelli, Alejandro R. PhD; Ritchie, James C. PhD; Jortani, Saeed A. PhD* Author Information *Department of Pathology and Laboratory Medicine, University of Louisville School of Medicine, Louisville, Kentucky Department of Pharmaceutical Sciences, St Jude Children's Research Hospital, Memphis, Tennessee Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia. The authors declare no conflicts of interest. Correspondence: Saeed A. Jortani, PhD, Department of Pathology and Laboratory Medicine University of Louisville School of Medicine, 511 South Floyd Street, Room 227 Louisville, KY 40202 (e-mail: [log in to unmask]). Received September 8, 2011 Accepted January 17, 2012 Abstract Background: The available assay kit for methotrexate (MTX) using the Syva enzyme multiplied immunoassay technique (EMIT) reagents on the Siemens Viva-E instrument allows for the detection of MTX in serum or plasma to concentrations as low as 0.3 µmole/L. Current clinical decision points for MTX therapeutic drug monitoring and leucorvorin rescue exist at concentrations below that limit. Objective: The goal of this study was to lower the limit of MTX quantitation to 0.05 µmole/L using the EMIT assay technology. Methods: EMIT MTX assay parameters were modified on the Viva-E instrument to increase the sample volume, alter the calibration method, and employ an alternate calibrator set created to achieve lower detection. Intraassay and interassay precision was assessed for MTX controls. Results: We observed a CV of 9.4% for intraassay precision with a bias of <0.01% and a CV of 15.7% for interassay precision with a bias of 22.5% for the 0.05 µmole/L control. Precision data for all other controls were <4%. The modified EMIT MTX assay and the unmodified approved assay were compared with a high sensitivity fluorescence polarization immunoassay method. Linear regression of correlation data revealed that both the modified and the commercial EMIT assays produced positive bias compared with the high sensitivity fluorescence polarization immunoassay method (y-int = 0.03 and 0.08, respectively). However, the modified EMIT assay had the best correlation in the low range (0.032 µmole/L). Additionally, endogenous and chemical interference testing demonstrated that the modified assay was not affected to a clinically significant extent. Conclusions: The described modifications have enhanced the sensitivity of the Syva EMIT assay for MTX measurements down to 0.05 µmole/L with acceptable precision that can be used in clinical practice for monitoring MTX therapy. ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk<http://www.acb.org.uk/> Green Laboratories Work http://www.laboratorymedicine.nhs.uk<http://www.laboratorymedicine.nhs.uk/> List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ Privacy and Confidentiality Notice: The information contained in this e-mail is intended for the named recipient(s) only. It may contain privileged and confidential information. If you are not an intended recipient, you must not copy, distribute or take any action in reliance on it. If you have received this e-mail in error, we would be grateful if you would notify us immediately. Thank you for your assistance. Please note that e-mails sent or received by our staff may be disclosed under the Freedom of Information Act (unless exempt). ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ######################################################################### Attention: This e-mail and any attachment is for authorised use by the intended recipient(s) only. It may contain proprietary, confidential and/or privileged information and should not be copied, disclosed, distributed, retained or used by any other party. If you are not an intended recipient please notify the sender immediately and delete this e-mail (including attachments and copies). The statements and opinions expressed in this e-mail are those of the author and do not necessarily reflect those of the Royal Marsden NHS Foundation Trust. The Trust does not take any responsibility for the statements and opinions of the author. Website: http://www.royalmarsden.nhs.uk ######################################################################### ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/