Hi Mat,I have to separate the b0 value from my dti adquisition in order to get a single 3D nifti volume containing the volume of DWI for the 0 b-value....I know I have to use flirt, but I don't know how to manage to calculate and apply a transformation to each of the volumes of 4D DTI sequences of my subjects.... :(I have been searching at the fsl mailing list but I had not been lucky.Please, could you help me with this question?Thank you very much.Rosalia.