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Dear FSL experts, I have subjects with two dti 4D adquisitions (dti1 and dti2 with 31 sequences). I have a question about how to split my 4D dti1 data into 3D in order to get a single 3D nifti volume containing the volume of DWI *for b0 value*....so, I should co-register volumes within dti1 to b0 value as the reference volume. I have been looking at applyxfm 4D usage with Flirt in the mailing list ...and I think it could be the best option for my objective [rosalia@UB17-336-161116099027 ~]$ applyxfm4D Usage: applyxfm4D <input volume> <ref volume> <output volume> <transformation matrix file/[dir]> [-singlematrix/-fourdigit/-userprefix <prefix>]] [rosalia@UB17-336-161116099027 ~]$ *But I am unsure about how to fix this with my subjects....* * * *Any example of suggestion about this issue?* Thanks in advance, Rosalia. 2012/4/21 Rosalia Dacosta <[log in to unmask]> > Hi Mat, > > I have to separate the b0 value from my dti adquisition in order to get a > single 3D nifti volume containing the volume of DWI for the 0 b-value....I > know I have to use flirt, but I don't know how to manage to calculate and > apply a transformation to each of the volumes of 4D DTI sequences of my > subjects.... :( > > I have been searching at the fsl mailing list but I had not been lucky. > > Please, could you help me with this question? > > Thank you very much. > > Rosalia. >