Hi all,
I've been using a Belle technologies anaerobic glovebox with in
built microscope and liquid nitrogen dewar port in an attempt to
harvest and cryo-cool crystals in an oxygen free environment.
I've been having some problems with icing and think this is most
likely due to slow speed that it is possible to plunge loops into
the liquid nitrogen. I've been plunging loops straight into vials
preloaded into an ESRF puck (with pusher). This requires more care
than what I would ordinarily do (i.e. plunging into bulk liquid
nitrogen then and manouvering into a vial afterwards and then
clipping said vial into a cane) and so takes longer - perhaps
leading to icing. The latter technique is not available as the
glovebox set up means that the dewar can only be reached by my right
hand - the microscope is in the way!
Does anyone have any experience of a similar set up and if so have
some advice as to how I can overcome this problem?
Many thanks,
Dave Gallagher
--
David Gallagher
PhD Student
MRC Mitochondrial Biology Unit
Wellcome Trust / MRC Building
Hills Road
Cambridge
CB2 0XY
Email:
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Phone: 01223 252913