On Thu, 2012-04-19 at 02:26 +0100, Krithika Sundaram wrote:
> Hi all,
>
> I am working on an oxidoreductase and having some trouble during molecular replacement.
>
> The resolution of the crystal is 1.7 A and the space group is I4122 (a = b = 121.086, c =156.93 and alpha = beta = gamma = 90).
>
> The cell content analysis results predicted two molecules in the asymmetric unit and the solvent content as 36 %. The Matthew's coefficient is 1.94. The Wilson plot also looks fine.
>
> However, after molecular replacement (using Phaser) the result just gives me just a single molecule. ( RFZ = 8.5 TFZ =13.9 PAK = 0 LLG =189 TFZ=18.0 LLG = 642)
>
> Any suggestions how to solve this problem?
>
> Thanks in advance.
>