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Have you tried the MiTeGen Micromesh? They're my favorite for handling tiny crystals. Ho Ho Leung Ng University of Hawaii at Manoa Assistant Professor, Department of Chemistry [log in to unmask] On Sat, Apr 7, 2012 at 1:00 PM, CCP4BB automatic digest system <[log in to unmask]> wrote: > > Hi all. > > I have small and fragile membrane protein crystals with size 40 micron. They like to break when I try take them out with regular loops for freezing. Is there any special tricks to handle them for data collection? > > Thank you. > > Theresa