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Is there room in the LN2 to plunge directly in, then maneuver into puck? That's what I did, with a different setup, but still anaerobic and moving into pucks. I did not run into ice problems. If there is not room within the LN2 bowl, can you use a larger one? I've found any hesitation when heading into LN2 (i.e.increased time spent hovering over LN2 in the gas/semi cool area) can cause ice. Thus I always try to plunge in quickly, then maneuver to the desired storage location (puck, cap). Kelly ******************************************************* Kelly Daughtry, Ph.D. Post-Doctoral Fellow, Raetz Lab Biochemistry Department Duke University Alex H. Sands, Jr. Building 303 Research Drive RM 250 Durham, NC 27710 P: 919-684-5178 ******************************************************* On Tue, Apr 24, 2012 at 10:20 AM, David Gallagher <[log in to unmask]>wrote: > Hi all, > > I've been using a Belle technologies anaerobic glovebox with in built > microscope and liquid nitrogen dewar port in an attempt to harvest and > cryo-cool crystals in an oxygen free environment. > > I've been having some problems with icing and think this is most likely > due to slow speed that it is possible to plunge loops into the liquid > nitrogen. I've been plunging loops straight into vials preloaded into an > ESRF puck (with pusher). This requires more care than what I would > ordinarily do (i.e. plunging into bulk liquid nitrogen then and manouvering > into a vial afterwards and then clipping said vial into a cane) and so > takes longer - perhaps leading to icing. The latter technique is not > available as the glovebox set up means that the dewar can only be reached > by my right hand - the microscope is in the way! > > Does anyone have any experience of a similar set up and if so have some > advice as to how I can overcome this problem? > > Many thanks, > > Dave Gallagher > > > -- > *David Gallagher * > PhD Student > > MRC Mitochondrial Biology Unit > Wellcome Trust / MRC Building > Hills Road > Cambridge > CB2 0XY > > Email: [log in to unmask] > Phone: 01223 252913 >