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Dear John, Thanks for the helpful answers and clarifying my confusion about the ICBM file. Just one more quick question and hope you don't mind answering it: Can you explain how the EPI template was generated in the current SPM8 version? -Glen On Fri, Mar 9, 2012 at 9:05 AM, John Ashburner <[log in to unmask]> wrote: > > I'm about to normalize 10 individual subjects' Beta maps onto the MNI > > standard template (/template/EPI.nii) and want to make sure that I'm > doing > > it correctly. > > So, I always use the mean image (that is generated after registration) as > > the source image when I normalize the bold images. And now, I'm > wondering if > > I can use the same mean image for normalizing Beta maps as well? > > The same spatial transform should be applicable to all data that is in > alignment with the image that you estimate the transform from. In > your case, the beta maps should be perfectly aligned with the mean > from the realignment, so the transform should be applicable. > > > > Also, as a side note, can anybody confirm that SPM8 still uses the MNI152 > > template for its template? In other words, /template/EPI.nii is the ICBM > 152 > > template, correct? > > The EPI.nii file is not actually an ICBM file. It was one that I > generated from a relatively small number of subjects scanned in the > FIL, using some quite old SPM functions (maybe SPM99, but I don't > remember now). It should be in approximate alignment with the ICBM > 152 data though. > > Best regards, > -John >