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Dear Alisha, The best instructions on how to add unusual amino acids are on our documentation wiki, at http://sites.google.com/site/ccpnwiki/Home/documentation/ccpnmr-analysis/howtos-1/create-a-sequence-with-unusual-amino-acids-or-nucleotides When you are on the 'Small Compounds' tab, you need to find the amino acid you need. The best way is to set the Mol Type pulldown to 'All', and use Right-Mouse:Filter with some suitable expressions to look for appropriate molecules. Filtering on 'diaminoburyric' gives you Dab (2,4 DiaminoButyric Acid), which is presumably what you want. Filtering on 'nor' gives you nothing better than Nnh (Nor-N-omega-hydroxy-L-Arginine). At a pinch you could use that one for assignment. To get just nor-L-Arginine (presumably what you want?) you would need to use ChemBuild, as others have recommended. The message about compounds not being found locally is a non-problem (but many find it confusing even so). If you have internet access, the popup says: "Chemical component not available locally. OK to download to project directory?" When you click OK it downloads the ChemComp you need. You then get a popup saying "Chemical component <ccpCode> available, but no atom coordinates, so a structure cannot be drawn." From which it follows that the ChemComp is ther, you can add it to your molecule, but it can just not display it, not having a set of example coordinates. Hope this helps, Rasmus --------------------------------------------------------------------------- Dr. Rasmus H. Fogh Email: [log in to unmask] Dept. of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002 On Fri, 16 Mar 2012, SUBSCRIBE CCPNMR Alisha Jones wrote: > Howdy, > > I have two nonstandard amino acids in my peptide sequence; norarginine > and diaminobutyric acid. When I enter my sequence into ccpnmr and try > to add dab, it says that it isn't found locally. How do I get around > this? Also, is there a way for me to create norarginine? I have just > used arginine and lysine for my assignments, respectively, seeming as > the two nonstandards are derivatives of those. Any insight would be > great. > > Thanks, > Alisha >