Dear Dipankar,

It just occurred to me that your high Rfactors may also be due to a large conformational change of your protein. In that case you have to split your search model in separate pdb files for the separate domains and rund Molrep with these separate domains.

Best,

Herman

From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Dipankar Manna
Sent: Wednesday, March 14, 2012 10:26 AM
To: [log in to unmask]
Subject: [ccp4bb] How to reduce R factor

Dear Crystallographers,

Can anybody guide me how to reduce R-factor, means which are the basic parameters I have to look for to reduce the R-factor during refinement. I am newly learning the refinement. After running molrep R-factor is around 53% (100% identity), after rigid body refinement its showing around 49% and after restrained refinement its showing around 47%. Highest resolution is 2.5A.

Regards

Dipankar

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