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We are trying to deal with a eukaryotic membrane protein that crystallizes but, as usual, diffracts poorly so far. As it is heavily glycosylated, we are considering enzymatic deglycosylation.

Does anyone know about a recombinant source of N-glycanase ; we would like to prepare it ourselves because the commercial sources are apparently too expensive.

Thanks in advance.
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Pascal F. Egea, PhD
Assistant Professor
UCLA, David Geffen School of Medicine
Department of Biological Chemistry
Boyer Hall room 356