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Hi all,
Has anyone used sodium acetate buffer pH (4-5) for purifying histag protein on Histrap column (AKTA) followed by SEC?
My protein has a pI of 9. I tried pH7.4 but it has precipitation problems. While doing buffer screening using 24 well hanging drop I found that lower pI onces are clear, so just thinking can I use Na acetate at pH 5 for whole purification???


 Thanks in advance
 Anita