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Hi, 1. You don't mention how many Zn sites you have, or how big your protein is - as Phil mentioned, these are factors. 2. I'll add to the chorus - pick your wavelength(s) based on a fluorescence scan. 3. If "1.5A0" is your wavelength, not your resolution, you may still have some anomalous signal - I've had a dataset collected on a copper rotating anode (wavelength 1.54) with detectable zinc anomalous signal. You'll get better signal closer to the anomalous peak; but if your Zn/protein ratio is large enough it may not be necessary. Pete Deepthi wrote: > Hi > > I am trying to solve the structure of an engineered protein.The protein is crystallized with Zn bound to it .We collected a 1.5A0 data. Molecular Replacement didn't yield a good match for the protein. I want to try MAD taking advantage of the Zn atoms in protein. I am not sure what wavelength should i use to collect the diffraction data for Zn. any suggestions? > > Thank You > Deepthi > > -- > Deepthi >