Dialing,<div><br><br><div class="gmail_quote"><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div><div style="font-size:12pt;font-family:times new roman,new york,times,serif">
<div><span>After
 we refine the structure of the protein to satisfactory with 
satisfactory Rwork and Rfree, we pick water by phenix refine, and I find
 <var></var>Rfree always increases slightly after the water picking refinment. </span></div><div><span></span> </div><div><span>Do you have nay idea to solve this problem or any comment?</span></div></div></div></blockquote>
<div><br></div><div>Just a reminder: there is phenixbb mailing list for Phenix related questions.</div><div><br></div><div>Replying to your post: phenix.refine uses very sophisticated criteria for water update (which doen&#39;t mean there is no room for further improvement of course). If you believe it placed or removed a wrong water please submit a bug report to me that should include:</div>
<div>1) all input files (reflection data, PDB file with the model, CIF ligand and parameter files if any);</div><div>2) the command you used to run the program;</div><div>3) explain which waters you believe are wrong.</div>
<div><br></div><div>Pavel.</div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div><div style="font-size:12pt;font-family:times new roman,new york,times,serif"><div>
<span style="font-size:12pt"> </span></div></div></div></blockquote></div><br></div>